Kontakt

Katrin  Sellrie
Katrin Sellrie
Telefon:+49 30 838-59572
Prof. Dr.  Peter H. Seeberger
Prof. Dr. Peter H. Seeberger
Direktor
Telefon:+49 331 567-9300Fax:+49 331 567-9302

Scientists of following industrial and academic institutions have used our facility:

RKI

MDC

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MPI – Glycan Array Screening Facility

Glycan array analysis is now standard technology of modern glycobiology. Complex carbohydrate structures are covalenly immobilized on a glass slide, which can subsequently be probed with potential interaction partners, for example lectins or antibodies. Glycan arrays have broad applications ranging from basic studies into the specificity of glycan binding proteins to medical research to identify biomarkers and aid vaccine development. The “Max Planck Society Glycan Array Screening Platform” is based mainly on synthetic oligosaccharides covering diverse glycan structures found throughout nature including but not limited to bacterial surface glycans, plant derived structures and cellular recognition motifs.

Our glycan array assays can be used for rapid screening to identify binding partners or evaluate the specificity of newly developed affinity probes. Tailor-made arrays can be printed to meet project needs including all quality controls. In house specialists On request can provide detailed analyses of the results.

More information concerning the Application Of Glycan Arrays can be found in the publications listed below:

1.
Geißner, A.; Anish, C.; Seeberger, P. H.: Glycan arrays as tools for infectious disease research. Current Opinion in Chemical Biology 18, S. 38 - 45 (2014)
2.
Geissner, A.; Seeberger, P. H.: Glycan Arrays: From Basic Biochemical Research to Bioanalytical and Biomedical Applications. Annual Review of Analytical Chemistry 9 (1), S. 223 - 247 (2016)
3.
Geißner, A.; Reinhardt, A.; Rademacher, C.; Johannssen, T.; Monteiro, J.; Lepenies, B.; Thépaut, M.; Fieschi, F.; Mrázková, J.; Wimmerova, M. et al.; Schuhmacher, F.; Götze, S.; Grünstein, D.; Guo, X.; Hahm, H. S.; Kandasamy, J.; Leonori, D.; Martin, C. E.; Parameswarappa, S. G.; Pasari,, S.; Schlegel, M. K.; Tanaka, H.; Xiao, G.; Yang, Y.; Pereira, C. L.; Anish, C.; Seeberger, P. H.: Microbe-focused glycan array screening platform. Proceedings of the National Academy of Sciences of the United States of America 116 (6), S. 1958 - 1967 (2019)

We routinely use glycan arrays for different applications. A selection of recent original research articles are listed below. Please contact us with a project proposal if you are interested in access to our screening platform.

2018

4.
Menova, P.; Sella, M.; Sellrie, K.; Pereira, C. L.; Seeberger, P. H.: Identification of the Minimal Glycotope of Streptococcus pneumoniae 7F Capsular Polysaccharide using Synthetic Oligosaccharides. Chemistry – A European Journal 24 (16), S. 4181 - 4187 (2018)

2017

5.
Lisboa, M. P.; Khan, N.; Martin, C.; Xu, F.-F.; Reppe, K.; Geissner, A.; Govindan, S.; Witzenrath, M.; Pereira, C. L.; Seeberger, P. H.: Semisynthetic glycoconjugate vaccine candidate against Streptococcus pneumoniae serotype 5. Proceedings of the National Academy of Sciences of the United States of America 114 (42), S. 11063 - 11068 (2017)
6.
Ruprecht, C.; Bartetzko, M.; Senf, D.; Dallabernardina, P.; Boos, I.; Andersen, M. C. F.; Kotake, T.; Knox, J. P.; Hahn, M. G.; Clausen, M. H. et al.; Pfrengle, F.: A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology 175 (3), S. 1094 - 1104 (2017)
7.
Hanske, J.; Wawrzinek, R.; Geissner, A.; Wamhoff, E.-C.; Sellrie, K.; Schmidt, H.; Seeberger, P. H.; Rademacher, C.: Calcium-independent activation of an allosteric network in Langerin by heparin oligosaccharides. ChemBioChem: A European Journal of Chemical Biology 18 (13), S. 1183 - 1187 (2017)

2016

8.
Parameswarappa, S. G.; Reppe, K.; Geißner, A.; Ménová, P.; Govindan, S.; Calow, A. D. J.; Wahlbrink, A.; Weishaupt, M. W.; Monnanda, B. P.; Bell, R. et al.; Pirofski, L.-A.; Suttorp, N.; Sander, L.; Witzenrath, M.; Pereira, C. L.; Chakkumkal, A.; Seeberger, P. H.: A Semi-synthetic Oligosaccharide Conjugate Vaccine Candidate Confers Protection against Streptococcus pneumoniae Serotype 3 Infection. Cell Chemical Biology 23 (11), S. 1407 - 1416 (2016)
9.
Geißner, A.; Pereira, C. L.; Leddermann, M.; Chakkumkal, A.; Seeberger, P. H.: Deciphering Antigenic Determinants of Streptococcus pneumoniae Serotype 4 Capsular Polysaccharide using Synthetic Oligosaccharides. ACS Chemical Biology 11 (2), S. 335 - 344 (2016)
10.
Broecker, F.; Hanske, J.; Martin, C. E.; Baek, J. Y.; Wahlbrink, A.; Wojcik, F.; Hartmann, L.; Rademacher, C.; Chakkumkal, A.; Seeberger, P. H.: Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans. Nature Communications 7, 11224 (2016)
11.
Bröcker, F.; Martin, C. E.; Wegner, E.; Mattner, J.; Baek, J. Y.; Pereira, C. L.; Chakkumkal, A.; Seeberger, P. H.: Synthetic Lipoteichoic Acid Glycans Are Potential Vaccine Candidates to Protect from Clostridium difficile Infections. Cell Chemical Biology 23 (8), S. 1014 - 1022 (2016)
12.
Reinhardt, A.; Wehle, M.; Geißner, A.; Crouch, E. C.; Kang, Y.; Yang, Y.; Chakkumkal, A.; Santer, M.; Seeberger, P. H.: Structure binding relationship of human surfactant protein D and various lipopolysaccharide inner core structures. Journal of Structural Biology 195 (3), S. 387 - 395 (2016)

2015

13.
Schmidt, D.; Schuhmacher, F.; Geissner, A.; Seeberger, P. H.; Pfrengle, F.: Automated Synthesis of Arabinoxylan-Oligosaccharides Enables Characterization of Antibodies that Recognize Plant Cell Wall Glycans. Chemistry – A European Journal 21 (15), S. 5709 - 5713 (2015)
14.
Pereira, C. L.; Geißner, A.; Chakkumkal, A.; Seeberger, P. H.: Chemical Synthesis Elucidates the Immunological Importance of a Pyruvate Modification in the Capsular Polysaccharide of Streptococcus pneumoniae Serotype 4. Angewandte Chemie, International Edition in English 54, S. 10016 - 10019 (2015)

2014

15.
Götze, S.; Azzouz, N.; Tsai, Y.-H.; Groß, U.; Reinhardt, A.; Anish, C.; Seeberger, P. H.; Silva, D. V.: Diagnosis of Toxoplasmosis Using a Synthetic Glycosylphosphatidylinositol Glycan. Angewandte Chemie, International Edition in English 53 (50), S. 13701 - 13705 (2014)
16.
Mietzsch, M.; Broecker, F.; Reinhardt, A.; Seeberger, P. H.; Heilbronn, R.: Differential Adeno-Associated Virus Serotype-Specific Interaction Patterns with Synthetic Heparins and Other Glycans. Journal of Virology 88 (5), S. 2991 - 3003 (2014)
17.
Maglinao, M.; Eriksson, M.; Schlegel, M. K.; Zimmermann, S.; Johannssen, T.; Götze, S.; Seeberger, P. H.; Lepenies, B.: A platform to screen for C-type lectin receptor-binding carbohydrates and their potential for cell-specific targeting and immune modulation. Journal of Controlled Release 175, S. 36 - 42 (2014)
 
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