MPI – Glycan Array Screening Facility
Glycan array analysis is now standard technology of modern glycobiology. Complex carbohydrate structures are covalenly immobilized on a glass slide, which can subsequently be probed with potential interaction partners, for example lectins or antibodies. Glycan arrays have broad applications ranging from basic studies into the specificity of glycan binding proteins to medical research to identify biomarkers and aid vaccine development. The “Max Planck Society Glycan Array Screening Platform” is based mainly on synthetic oligosaccharides covering diverse glycan structures found throughout nature including but not limited to bacterial surface glycans, plant derived structures and cellular recognition motifs.
Our glycan array assays can be used for rapid screening to identify binding partners or evaluate the specificity of newly developed affinity probes. Tailor-made arrays can be printed to meet project needs including all quality controls. In house specialists On request can provide detailed analyses of the results.
More information concerning the Application Of Glycan Arrays can be found in the publications listed below:
Andreas Geißner, Chakkumkal Anish, and Peter H. Seeberger, "Glycan arrays as tools for infectious disease research," Current Opinion in Chemical Biology 18, 38-45 (2014).
Andreas Geissner and Peter H. Seeberger, "Glycan Arrays: From Basic Biochemical Research to Bioanalytical and Biomedical Applications.," Annual Review of Analytical Chemistry 9 (1), 223-247 (2016).
We routinely use glycan arrays for different applications. A selection of recent original research articles are listed below. Please contact us with a project proposal if you are interested in access to our screening platform.
Petra Menova, Mauro Sella, Katrin Sellrie, Claney L. Pereira, and Peter H. Seeberger, "Identification of the Minimal Glycotope of Streptococcus pneumoniae 7F Capsular Polysaccharide using Synthetic Oligosaccharides," Chemistry – A European Journal 24 (16), 4181-4187 (2018).
Marilda P. Lisboa, Naeem Khan, Christopher Martin, Fei-Fei Xu, Katrin Reppe, Andreas Geissner, Subramanian Govindan, Martin Witzenrath, Claney L. Pereira, and Peter H. Seeberger, "Semisynthetic glycoconjugate vaccine candidate against Streptococcus pneumoniae serotype 5," Proceedings of the National Academy of Sciences of the United States of America 114 (42), 11063-11068 (2017).
Colin Ruprecht, Max Bartetzko, Deborah Senf, Pietro Dallabernardina, Irene Boos, Mathias C. F. Andersen, Toshihisa Kotake, J. Paul Knox, Michael G. Hahn, Mads H Clausen, and Fabian Pfrengle, "A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies," Plant Physiology 175 (3), 1094-1104 (2017).
Jonas Hanske, Robert Wawrzinek, Andreas Geissner, Eike-Christian Wamhoff, Katrin Sellrie, Henrik Schmidt, Peter H. Seeberger, and Christoph Rademacher, "Calcium-independent activation of an allosteric network in Langerin by heparin oligosaccharides," ChemBioChem: A European Journal of Chemical Biology 18 (13), 1183-1187 (2017).
Sharavathi Guddehalli Parameswarappa, Katrin Reppe, Andreas Geißner, Petra Ménová, Subramanian Govindan, Adam D. J. Calow, Annette Wahlbrink, Markus W. Weishaupt, Bopanna Ponnappa Monnanda, Roland Lawrence Bell, Liise-Anne Pirofski, Norbert Suttorp, Leif Erik Sander, Martin Witzenrath, Claney Lebev Pereira, Anish Chakkumkal, and Peter H. Seeberger, "A Semi-synthetic Oligosaccharide Conjugate Vaccine Candidate Confers Protection against Streptococcus pneumoniae Serotype 3 Infection," Cell Chemical Biology 23 (11), 1407-1416 (2016).
Andreas Geißner, Claney Lebev Pereira, Melanie Leddermann, Anish Chakkumkal, and Peter H. Seeberger, "Deciphering Antigenic Determinants of Streptococcus pneumoniae Serotype 4 Capsular Polysaccharide using Synthetic Oligosaccharides," ACS Chemical Biology 11 (2), 335-344 (2016).
Felix Broecker, Jonas Hanske, Christopher E. Martin, Ju Yuel Baek, Annette Wahlbrink, Felix Wojcik, Laura Hartmann, Christoph Rademacher, Anish Chakkumkal, and Peter H. Seeberger, "Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans," Nature Communications 7, 11224 (2016).
Felix Bröcker, Christopher E. Martin, Erik Wegner, Jochen Mattner, Ju Yuel Baek, Claney L. Pereira, Anish Chakkumkal, and Peter H. Seeberger, "Synthetic Lipoteichoic Acid Glycans Are Potential Vaccine Candidates to Protect from Clostridium difficile Infections," Cell Chemical Biology 23 (8), 1014-1022 (2016).
Anika Reinhardt, Marko Wehle, Andreas Geißner, Erika C. Crouch, Yu Kang, You Yang, Anish Chakkumkal, Mark Santer, and Peter H. Seeberger, "Structure binding relationship of human surfactant protein D and various lipopolysaccharide inner core structures," Journal of Structural Biology 195 (3), 387-395 (2016).
Deborah Schmidt, Frank Schuhmacher, Andreas Geissner, Peter H. Seeberger, and Fabian Pfrengle, "Automated Synthesis of Arabinoxylan-Oligosaccharides Enables Characterization of Antibodies that Recognize Plant Cell Wall Glycans," Chemistry – A European Journal 21 (15), 5709-5713 (2015).
Claney Lebev Pereira, Andreas Geißner, Anish Chakkumkal, and Peter H. Seeberger, "Chemical Synthesis Elucidates the Immunological Importance of a Pyruvate Modification in the Capsular Polysaccharide of Streptococcus pneumoniae Serotype 4," Angewandte Chemie, International Edition in English 54, 10016-10019 (2015).
Sebastian Götze, Nahid Azzouz, Yu-Hsuan Tsai, Uwe Groß, Anika Reinhardt, Chakkumkal Anish, Peter H. Seeberger, and Daniel Varon Silva, "Diagnosis of Toxoplasmosis Using a Synthetic Glycosylphosphatidylinositol Glycan," Angewandte Chemie, International Edition in English 53 (50), 13701-13705 (2014).
Mario Mietzsch, Felix Broecker, Anika Reinhardt, Peter H. Seeberger, and Regine Heilbronn, "Differential Adeno-Associated Virus Serotype-Specific Interaction Patterns with Synthetic Heparins and Other Glycans," Journal of Virology 88 (5), 2991-3003 (2014).
M. Maglinao, M. Eriksson, Mark K. Schlegel, Stephanie Zimmermann, Timo Johannssen, Sebastian Götze, Peter H. Seeberger, and Bernd Lepenies, "A platform to screen for C-type lectin receptor-binding carbohydrates and their potential for cell-specific targeting and immune modulation," Journal of Controlled Release 175, 36-42 (2014).