Ribosomes are complex molecular machines that translate the codon
sequences of mRNA molecules into amino acid sequences, the primary structure
of proteins. This translation process
involves numerous ribosomal states and individual transitions that can be studied in vitro
but not in vivo. A general computational method has been recently developed by which
one can deduce the in-vivo rates from their in-vitro values
[1]. The deduced
rates have been validated by three independent sets of in-vivo data.
Other interesting aspect sof translation are provided by the formation of polysomes, i.e.,
the simultaneous translation of the same mRNA by several ribosomes and by the
relatively short life time of the mRNA
[2].
This aging effect leads to translation rates that decrease with increasing mRNA length
[3].
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S. Rudorf, M. Thommen, M. Rodnina, and R. Lipowsky
Deducing the kinetics of protein synthesis in vivo from
the transition rates measured in vitro.
Deducing the kinetics of protein synthesis in vivo from
... - Supporting Information.
PLoS Comp. Biol. 10, e1003909 (2014).
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C. Deneke, R. Lipowsky and A. Valleriani
Effect of ribosome shielding on mRNA stability.
Phys. Biol. 10, 046008 (2013).
-
A. Nagar, A. Valleriani, and R. Lipowsky
Translation by ribosomes with mRNA degradation: Exclusion
processes on aging tracks.
J. Stat. Phys. 145, 1385-1404 (2011).
-
A. Valleriani, Gong Zhang, A. Nagar, Z. Ignatova, and R. Lipowsky
Length dependent translation of messenger RNA by ribosomes.
Phys. Rev. E 83, 042903 (2011).
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A. Valleriani, Z. Ignatova, A. Nagar, and R. Lipowsky
Turnover of messenger RNA: Polysome statistics beyond
the steady state.
EPL 89, 58003 (2010).
