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Table of content
Part I: The making of
Preparation methods for giant unilamellar vesicles
Rumiana Dimova, Pasquale Stano, Carlos M. Marques and Peter Walde
Giant unilamellar vesicles (GUVs). Methods based on vesicle swelling on substrates. Methods based on assembly from fluid interfaces. Dealing with lipids. Which method to choose?
Preparation and properties of giant plasma membrane vesicles and giant unilamellar vesicles from natural membranes
Joseph H. Lorent and Ilya Levental
Introductory words. Complexity of biological membranes. Giant plasma membrane vesicles (GPMVs). Preparation of GPMVs. Preparation of GUVs from natural membranes. Red blood cell ghosts. Rafting into the future.
Protein reconstitution in giant vesicles
Matthias Garten, Daniel Lévy and Patricia Bassereau
Introduction. Reconstitution of membrane proteins in small unilamellar vesicles (proteoliposomes).
Giant vesicles prepared from proteoliposomes by dehydration/rehydration techniques.
GUVs with cytoskeleton
Tobias Härtel and Petra Schwille
Next level of complexity in a bottom-
Part II: Giant vesicles theoretically and in silico
Understanding giant vesicles – a theoretical perspective
Introduction and overview. Basic aspects of biomembranes and multi-
Simulating membranes, vesicles, and cells
Thorsten Auth, Dmitry A. Fedosov and Gerhard Gompper
Introduction. Membrane models and simulation techniques. Applications. Conclusions and outlook.
Theory of vesicle dynamics in flow and electric fields
Petia M. Vlahovska and Chaouqi Misbah
Introduction. Problem formulation. Asymptotic solution for small deformations. Examples: a quasi–spherical vesicle in external fields. Outlook.
Introduction and overview. Different processes induced by particle-
Theory of polymer-
Fabrice Thalmann and Carlos M. Marques
Bilayers and polymers are intimate old friends. How do polymers interact with membranes?
Membranes in a solution of depleted nanoparticles: rods and spheres. Membranes in
solutions of non-
Part III: GUV-
Application of optical microscopy techniques on giant unilamellar vesicles
Luis A. Bagatolli
Mechanics assays of synthetic lipid membranes based on micropipette aspiration
Elisa Parra and David Needham
Historical overview. Experimental setup for micropipette manipulation. Micromechanics of single giant unilamellar vesicles. Molecular exchange and interactions between pairs of vesicles. Outlook: conclusions and combination between the micropipette technique and other approaches.
Atomic force microscopy of giant unilamellar vesicles
Introduction. Atomic force microscopy. Contact mechanics. Membrane mechanics. Modeling GUV mechanics probed with an AFM. Indentation of sessile GUVs. Conclusions and outlook.
Manipulation and biophysical characterization of GUVs with an optical stretcher
Gheorghe Cojoc, Antoine Girot, Ulysse Delabre and Jochen Guck
Introductory words. Optical stretching basics. Building an optical stretcher. Deformation of vesicles without heating: mechanical investigation. Deformation of vesicles with heating: thermodynamic investigation. Discussion. Outlook.
Vesicle fluctuation analysis
John Hjort Ipsen, Allan Grønhøj Hansen and Tripta Bhatia
Introduction. The measurement. Estimation of the correlation function. Applications. Discussion and conclusions.
Using electric fields to assess membrane material properties in GUVs
Rumiana Dimova and Karin A. Riske
Introductory words. Some equations. How to measure membrane properties by exposing GUVs to electric fields. Assessing overall vesicle properties and manipulation of GUVs. Experimental chambers for studying GUVs exposed to electric fields and tips for successful experiments. Final words.
Creating membrane nanotubes from GUVs
Coline Prévost, Mijo Simunovic and Patricia Bassereau
Introduction. Physics of a membrane nanotube connected to a GUV. Pulling nanotubes with optical tweezers. Pulling nanotubes without optical tweezers. Past and potential future applications of nanotubes.
Measuring GUV adhesion
Kheya Sengupta and Ana Smith
Introductory words. Context. Ingredients. Measurement of shape. Accurate measure
of fluctuations. Imaging molecules and bonds. De-
Phase diagrams and tie lines in GUVs
Matthew C. Blosser, Caitlin Cornell, Scott P. Rayermann and Sarah L. Keller
Introduction. Identifying coexisting phases in GUVs. Identifying tie lines in GUVs. Experimental caveats. Parting thoughts.
Vesicle dynamics in flow: an experimental approach
Victor Steinberg and Michael Levant
Introduction. Dynamics of a vesicle in a linear flow. Vesicle dynamics in an elongation flow. Role of thermal noise in vesicle dynamics. Hydrodynamic interaction of vesicles and dynamics of vesicles under confinement. Outlook.
Membrane permeability measurements
Membrane permeability. Permeability to water. Permeability to other molecules. Pore forming proteins and peptides. Outlook: control over permeability.
Part IV: GUVs as membrane interaction platforms
Lipid and protein mobility in GUVs
Introduction. Selection of probes for studying membrane dynamics. Fluorescence correlation microscopy. Fluorescence recovery after photobleaching. Single molecule imaging. Future outlook.
Shining light on membranes
Rosangela Itri, Carlos M. Marques and Mauricio S. Baptista
Membranes and light. GUVs as a support to understand photoreceptors. Photoinduced
physical transformations. Photoinduced chemical transformations. Membranes and light
in medicine, dermo-
Eva M Schmid and Daniel A Fletcher
Introduction. Background. Experimental methods for investigating protein-
Effects of antimicrobial peptides and detergents on GUVs
Karin A. Riske
Introductory words. Experimental methodology. Mode of action of antimicrobial peptides. Solubilization of GUVs by detergents. Final words.
Introduction. Interactions between polymers and lipid membranes. Polymers affect membrane structures at different scales. GUVs with fluid membrane in interaction with LCST neutral polymers. GUVs with fluid membrane interacting with charged polymers. Vision.
Part V: GUVs as complex membrane containers
Praful Nair, David Christian and Dennis E. Discher
Introduction. Block copolymer amphiphiles & assemblies. General background of structures. Motivation for polymer approaches. Other common block copolymers. Methods for synthesizing polymers. Methods for making and observing pGUVs. Common types of experiments. Conclusions.
Giant hybrid polymer/lipid vesicles
Thi Phuong Tuyen Dao, Khalid Ferji, Fabio Fernandes, Manuel Prieto, Sébastien Lecommandoux,
Emmanuel Ibarboure, Olivier Sandre and Jean-
Introductory words. Criteria to be fulfilled to obtain hybrid giant vesicles. Specific aspects of the formation of giant hybrid unilamellar vesicles. Understanding membrane properties from membrane structure. Conclusion and perspectives.
Giant unilamellar vesicles: from protocell models to the construction of minimal cells
Masayuki Imai and Peter Walde
Introductory words. Fatty acid vesicles as protocell models. Self-
Encapsulation of aqueous two-
Allyson M. Marianelli and Christine D. Keating
Introductory words. Aqueous two-
Johannes P. Frohnmayer, Marian Weiss, Lucia T. Benk, Jan-
Introductory words. Formation, functionalization and characterization of microfluidic
droplets: necessary ingredients and equipment. Formation of droplet-
List of lipids and physical constants of lipid bilayers
List of membrane dyes and fluorescent groups conjugated to lipids
List of detergents
List of water-