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Dr. Amaia Cipitria
Dr. Amaia Cipitria
Emmy-Noether-Gruppenleiterin
Telefon:+49 331 567-9452Fax:+49 331 567-9402
Dr. Inés Moreno Jiménez
Humboldt Postdoctoral Researcher
Telefon:+49 331 567-9446Fax:+49 331 567-9402

MSc thesis: Chorioallantoic membrane (CAM) assay for the study of multiple myeloma

Stellenangebot vom 13. Januar 2020

Background: Biology, Biomedicine, Biotechnology or Biomedical Engineering.

Moreno-Jiménez et al (2017) Tissue Engineering Part C: Methods
Moreno-Jiménez et al (2017) Tissue Engineering Part C: Methods

Development of an alternative, less sentient in vivo model (CAM assay) to study the effect of multiple myeloma cancer cells in the bone matrix microenvironment.

Multiple myeloma (MM) is a type of blood cancer in which plasma cells grow aberrantly and produce abnormal antibodies. In the clinic, MM patients show lesions in the skeleton which do not heal, even when the patient is in complete remission. MM cells mainly reside in the bone marrow and alter bone homeostasis by activating bone resorption and inhibiting bone formation. We are interested in understanding how these cancer cells alter the biophysical properties of the bone extracellular matrix. The project will involve cell culture of human MM cell lines to study their interaction and effects with the extracellular matrix of embryonic chick femurs. In the first part of the project you will culture femurs from chick embryos in vitro (organotypically) together with MM cells. In a second part of the project you will use the chorioallantoic membrane (CAM) assay as a less sentient, refinement model (3Rs) to examine the interaction of cancer cells with the bone tissue in vivo. Exploratory analysis will involve histology to localize cancer cells in the tissue and micro computed tomography to evaluate 3D changes in the bone structure.

You should have a background in biology, biomedicine, biomedical engineering, biotechnology or similar, and strong interest in cancer biology. Previous experience with cell culture will be highly valuable, but not essential. The project will be carried out at the Max Planck Institute of Colloids and Interfaces in the group of Dr Amaia Cipitria together with Dr Inés Moreno.

Please send your application including a motivation letter, your CV and a transcript of your university record to these two email addresses: and amaia.cipitria@mpikg.mpg.de

Please indicate “Master thesis –CAM assay and multiple myeloma” in the subject line. The project can start at the beginning of March 2020. The working language is English.

Dr Amaia Cipitria (Emmy Noether Group Leader) and Dr Inés Moreno (Humboldt Postdoctoral researcher), Dept. of Biomaterials, Max Planck Institute of Colloids and Interfaces, Golm, Potsdam.

 
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