Highlights
News and Highlights:
Multi-laboratory Study for comparing different analytical glycomics Methods
Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines : HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.
Ito H, Kaji H, Togayachi A, Azadi P, Ishihara M, Geyer R, Galuska C, Geyer H, Kakehi K, Kinoshita M, Karlsson NG, Jin C, Kato K, Yagi H, Kondo S, Kawasaki N, Hashii N, Kolarich D, Stavenhagen K, Packer NH, Thaysen-Andersen M, Nakano M, Taniguchi N, Kurimoto A, Wada Y, Tajiri M, Yang P, Cao W, Li H, Rudd PM, Narimatsu H.
Glycoconj J. 2015 Oct 28. doi:10.1007/s10719-015-9625-3
How does Flagellin glycosylation influence its function?
Flagellin glycosylation in Paenibacillus alvei CCM 2051T.
Janesch B, Schirmeister F, Maresch D, Altmann F, Messner P, Kolarich D, Schäffer C.
Glycobiology. 2015 Sep 24. pii: cwv087. doi:10.1093/glycob/cwv087
Which role does E-Cadherin N-glycosylation play in gastric cancer?
Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer.
Carvalho S, Catarino TA, Dias AM, Kato M, Almeida A, Hessling B, Figueiredo J, Gärtner F, Sanches JM, Ruppert T, Miyoshi E, Pierce M, Carneiro F, Kolarich D, Seruca R, Yamaguchi Y, Taniguchi N, Reis CA, Pinho SS.
Oncogene. 2015 Jul 20. doi: 10.1038/onc.2015.225.
The "How to do" for PGC-LC ESI MS/MS based glycomics of N- und O-glycans.
Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.
Kolarich Daniel, Windwarder Markus, Alagesan Kathirvel, Altmann Friedrich.
Methods Mol Biol. 2015;1321:427-35. doi: 10.1007/978-1-4939-2760-9_29
How do glycopeptides "Fly" in the mass spec? Some intersting Answers to some basic questions.
Stavenhagen K, Hinneburg H, Thaysen-Andersen M, Hartmann L, Silva DV, Fuchser J, Kaspar S, Rapp E, Seeberger PH, Kolarich D.
Quantitative mapping of glycoprotein micro-heterogeneity and macro-heterogeneity: an evaluation of mass spectrometry signal strengths using synthetic peptides and glycopeptides
J Mass Spectrom. 2013 Jun;48(6):627-39. doi: 10.1002/jms.3210 [Link]
Overview on Methods and Approaches for glycomics and Glycoproteomics Research. Information and Protocols:
"Mass Spectrometry for Glycomics Analysis of N- and O-linked Glycoproteins" by Daniel Kolarich and Nicolle H. Packer, in "Structural Glycobiology", CRC Press, edited by Elizabeth Yuriev and Paul A. Ramsland, [Link]
Determination of site-specific glycan heterogeneity on glycoproteins
Kolarich D, Jensen PH, Altmann F, Packer NH.
Nat Protoc. 2012 Jun 7;7(7):1285-98. doi: 10.1038/nprot.2012.062
PMID: 22678432, [Link]
Structural analysis of N- and O-glycans released from glycoproteins
Jensen PH, Karlsson NG, Kolarich D, Packer NH.
Nat Protoc. 2012 Jun 7;7(7):1299-310. doi: 10.1038/nprot.2012.063
PMID: 22678433, [Link]
Glycomics, glycoproteomics and the immune system.
Kolarich D, Lepenies B, Seeberger PH.
Curr Opin Chem Biol. 2012 Apr;16(1-2):214-20. Review.
PMID: 22221852, [Link]