The book begins with an introduction to the fundamentals of electrospinning, discussing fundamental principles of the electrospinning process, controlling parameters, materials and structures. Nanofabrication strategies, including coaxial electrospinning, multi-needle electrospinning, needleless electrospinning, electro-netting, near-field electrospinning, and three-dimensional macrostructure assembling are also covered. Final sections explore the applications of electrospun nanofibers in different fields and future prospects.